5-Year Impact Factor: 0.9
Volume 34, 12 Issues, 2024
  Letter to the Editor     November 2024  

Detection of Hyperdiploidy from Cerebrospinal Fluid in B-Cell Acute Lymphoblastic Leukaemia

By Emine Goktas1, Sinan Demircioglu2

Affiliations

  1. Department of Medical Genetics, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkiye
  2. Department of Internal Medicine Division of Haematology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkiye
doi: 10.29271/jcpsp.2024.11.1394

Sir,

In B cell acute lymphoblastic leukaemia (B-ALL), the identification of chromosomal rearrangements has resulted in significant advancements in the prediction of prognosis, risk stratification, and the creation of targeted therapies. High hyperdiploidy, TEL-AML fusion, mixed lineage lekaemia (MLL) rearrangements, BCR-ABL1, t(1;19) (q23;p13), intrachromosomal amplification of chromosome 21 (iAMP21), and hypodiploidies are some of the genetic chromosomal changes observed in B-ALL.1 Genetic abnormalities can be detected in 75 to 80% of childhood ALL cases with standard chromosome analysis and interphase fluorescence in situ hybridisation (iFISH) analysis.2

A 14-year female patient with a diagnosis of B-ALL received  IC-BCF chemotherapy protocol. At the time of diagnosis, the cytogenetic and iFISH methods detected a hyperdiploid karyotype >50 at chromosomes 8, 10, 11, 14, 17, and 21. Four years later, the patient complained of neck swelling and was considered a relapse of ALL. At that time, the cytogenetic analysis detected hyperdiploid karyotype >50 at chromosomes 8, 11, 14, and 21 again. The patient achieved remission with the FLAG chemotherapy protocol and then allogeneic stem cell transplantation was performed after fludarabine+ATG+TBI+cyclopho-sphamide preparation regimen. About one year after the transplantation, the patient presented with complaints of dizziness, headache, and diplopia. The patient's bone marrow sample was analysed cytogenetically and with FISH analysis, and the results were normal. Cerebrospinal fluid (CSF) sample was taken from the patient whose bone marrow was in remission. In the CSF sample, CD10(+), CD19(+), CD20(+), CD22(+), Tdt(+), MPO(-), HLA-DR(+) CD34(-), and CALLA(+) were detected through flow cytometry. At the same time, cytogenetic and iFISH analyses were performed on the CSF sample. iFISH analysis detected hyperdiploid karyotype >50 at chromosomes 8, 11, 14, and 21 (Figure 1).

Additionally, the polymerase chain reaction (PCR) method was used to perform BCR-ABL1 fusion gene analysis on the CSF sample, and the result was negative. The patient, who was thought to have isolated central nervous system involvement, received intrathecal methotrexate, cytarabine and dexamethasone treatment. The patient's clinical findings completely improved in the first week after intrathecal treatment. No leukaemia cells were observed in CSF in the first month of treatment. Sebsequently, systemic chemotherapy was started with methotrexate 3.5 g/m2. Allogeneic stem cell transplantation was planned.

Figure 1: (A) Increased copy number of chromosome 21 or the AML1 gene region. (B) Trisomy of the c-myc gene region or chromosome 8.

In order to achieve an accurate diagnosis of CNS involvement in ALL, it is essential to adopt a comprehensive approach. Techniques such as immunocytochemistry, flow cytometry, PCR, and iFISH are used to detect any residual leukaemic cells in the CSF. The quantity and quality of CSF cells are the limiting factors for PCR, flow cytometry, and immunocytochemical examinations. However, the FISH method is a fast, and economical technique that can detect small amounts of residual ALL cells.3 In a study comparing cytology analysis and FISH analysis in CSF samples of ALL patients, approximately 9% of patients did not have sufficient cells for cytological examination. However, FISH analysis was still possible.4

In conclusion, CNS involvement was confirmed by performing FISH analysis on the CSF sample of an ALL patient whose bone marrow was in remission. Thus, the patient received the appropriate treatment and went into remission again quickly.

COMPETING  INTEREST:
The authors declared no conflict of interest.

AUTHORS’  CONTRIBUTION:
EG: Contributed to data collection, drafted the text, conducted the related literature search, and did reference setting.
SD: Contributed to data collection and interpreted the data.
Both authors approved the final version of the manuscript to be published.

REFERENCES

  1. Iacobucci I, Mullighan CG. Genetic basis of acute lymphoblastic leukemia. J Clin Oncol 2017; 35(9):975-83. doi: 10.1200/JCO.2016.70.7836.
  2. Magatha LS, Scott JX, Subramaniam G, Chandrasekaran T, Paul SFD, Koshy T. Cytogenetic and fluorescence in situ hybridization profile of pediatric acute lymphoblastic leukemia in a university hospital in South India. Med Princ Pract 2021; 30(6):563-70. doi: 10.1159/000518280.
  3. Karapetyan K, Gizhlaryan M, Kalinovskaia O, Hovhannisyan A, Tadevosyan G, Matinyan L, et al. Investigating residual leukemic cells in acute lymphoblastic leukemia: A practical approach using a streamlined interphase fluorescence in situ hybridization method on cerebrospinal fluid. Mol Cytogenet 2023; 16(1):17. doi: 10.1186/s13039-023-00 649-x.
  4. Hwang SM, Park HS, Park S, Kim S-M, Hong KT, Chang YH, et al. Application of fluorescence in situ hybridization on cerebrospinal fluid cytospins for the detection of residual leukemic cells in patients with childhood acute lympho-blastic leukemia. Am J Clin Pathol 2019; 151(4):416-23. doi: 10.1093/ajcp/aqy160.